Detritylation reagent
WebDec 8, 2024 · Although for most reagents, this is generally not an issue, extending the contact time of the detritylation reagent with oligodeoxynucleotides, which are sensitive to acid-based depurination and further strand cleavage during C&D, would lead to lower FLP (Full Length Product) purity (Fig. 5). WebHoneywell Burdick & Jackson™ Detritylation Reagent, Toluene with 10% Dichloroacetic Acid BioSyn™, for DNA/RNA synthesis Honeywell Burdick & Jackson™ Oxidation …
Detritylation reagent
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Web世联博研公司现货供应vwr品牌订货号为SR674-4的Trichloroacetic acid 3% in toluene, BioSyn™ Detritylation Reagent, for DNA/RNA synthesis, Burdick & Jackson™产品 联系方式: 400-650-8506 WebKeyword:'detritylation reagent' Showing 1-2 of 2 results for "detritylation reagent" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. …
WebJul 20, 2024 · Reagent delivery is encoded using pump strokes and flow rate. Each stroke of the HPLC pump carries 40 µL of solvent and it takes 12 strokes from both pumps (960 µL) to reach the resin. Web676 Detritylation Reagent (Honeywell Burdick & Jackson) CAS Number: 108-88-3 . Shop now or request a quote Shop Honeywell Burdick & Jackson™ Detritylation Reagent, ...
WebThe reactions of detritylation, coupling, oxidation/sulfurization, capping, and backbone deprotection employ a number of reagents and some of the more common solutions used are listed in Table 2. The physicochemical properties of these reagents vary, which could lead to different interactions with the solid support or the growing ...
WebDetritylation reagent 3% Dichloroacetic Acid in Dichloromethane (v/v), BioSyn™ for DNA/RNA synthesis, Burdick & Jackson™. Supplier: Honeywell Research Chemicals. …
WebDetritylation of the support-bound 3'-nucleoside. ... The reagent for the above phosphitylation reaction is made by treating phosphorus trichloride with 2-cyanoethanol then with N,N-diisopropylamine in the presence of DIPEA (N,N-diisopropylethylamine, a non-nucleophilic base; Figure 22). Other secondary amines can be attached to the … can cats retract all four clawsWebTrichloroacetic acid (TCA) 3% in dichloromethane, BioSyn™ detritylation reagent, for DNA/RNA synthesis, Burdick & Jackson™ Trichloroacetic acid (TCA) Reagents are … can cats remember where they liveWebAug 1, 1996 · DNA synthesis reagents. Acid binding experiments used DNA synthesized on high load CPG (100 µmol/g). The DNA sequences were based on the following standard … can cats scratch velvetWebChemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others. ... Honeywell Burdick & Jackson™ Detritylation Reagent, Toluene with 10% … can cats scratch glassWebRNA deprotection is unique because of the necessity to retain the 2' protecting group during cleavage and base deprotection. 2'-OMe-RNA and 2'-F-RNA, however, are virtually identical to DNA during deprotection. But, if a hybrid oligonucleotide contains even a single RNA linkage (with the exception of a 3'-ribonucleoside linkage), the oligo must ... fishing reel hard caseWebThis design eliminates the need topurify synthetic intermediates or unreacted reagents because they are simplyrinsed off the column at the end of each chemical step. ... Also, coupling efficiency and accuracy are increasedby this wash since premature detritylation of the incoming phosphoramiditemonomer is prevented. (2. ACTIVATION-COUPLING ... can cats scratch leather furnitureWebTraditionally dilute solutions of trichloroacetic acid (TCA, pK a 0.8) or dichloroacetic acid (DCA, p Ka 1.5) in dichloromethane (DCM) were used as detritylation reagent for ON synthesis. 125b, 132 Later, it was recognized that a high concentration of a weak acid (15% DCA in DCM) is more effective at driving detritylation toward completion than ... fishing reel left hand or right hand