Flag-tag mouse monoclonal antibody

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August undefined, 2024

WebWestern blot was performed using HRP-conjugated Anti-DYKDDDDK Mouse Monoclonal Antibody (Product # MA1-91878-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), HA-H3-FLAG, 40 µg (Lane 3), HA-H3-FLAG, 20 µg (Lane 4), HA … WebJun 15, 2012 · The current study reports the successful establishment of hybridoma cells that produce an extremely high-affinity antibody to FLAG, namely 2H8 Ab. 2H8 Ab …

His-Tag Monoclonal Antibody-博奥龙Biodragon

WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. WebJun 15, 2012 · The FLAG sequence (DYKDDDDK) is an artificial sequence widely used to detect, quantify, and purify proteins expressed as FLAG-fusion proteins. Several highly specific monoclonal antibodies for FLAG are commercially available; however, they are not always sensitive enough to detect proteins expressed … eastchester parking rules

DDDDK tag Monoclonal antibody (Binds to FLAG® tag …

WebDYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic … Web3 hours ago · Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, Burlington, MA, USA, 1:10.000 dilution) for 1 h at RT. ... or an established mouse IgG2 monoclonal antibody recognizing PSD95. In these indirect setups, after washing (3 × 5 min in PBS each), the anti-PSD95 r-hcAb or … Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M eastchester parking permit

A high-affinity monoclonal antibody against the FLAG tag

Flag-Tag Monoclonal Antibody-博奥龙Biodragon

WebMouse monoclonal [F-tag-01] antibody to DDDDK Tag. Applications: ICC, WB: Reactivity: Species independent: Conjugate: Unconjugated: ... Anti-FLAG Tag Antibody [FG4R] … WebFlag Tag Monoclonal Antibody Datasheet Product Details Citations Customer Reviews and Q&A Product Details Citations Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2024 eastchester parkingWebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized peptide. Western blot analysis of Flag-tagged protein expression in (1) 293T cell lysate; (2) 293T cell transfected with Flag-tagged protein lysate. β-Actin Rabbit Polyclonal Antibody eastchester parks and recreation

"WebDYKDDDDK Tag Monoclonal Antibody ... -Mouse Monoclonal Antibody Acquista DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™ su. Italiano English; Change Country. Ricevi tutte le ultime informazioni Centro di Assistenza e Supporto › Italiano English; Change Country; 0. No offer available; Struttura chimica ... " - Flag-tag mouse monoclonal antibody

Flag-tag mouse monoclonal antibody

DYKDDDDK Tag (9A3) Mouse mAb (Binds to same …

http://www.abpbiosciences.com/product/flag-tag/ WebHost Species Mouse; Clonality Monoclonal; Purification Affinity purification using immunogen. Applications WB,IF IP; Species Reactivity Hu Ms Rt; Specificity The Flag …

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WebMouse Antibody Monoclonal. Introduction. A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. … WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.

WebKey features and details PE Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt, Sandwich ELISA Reacts with: Species independent Conjugation: PE. Ex: 488nm, Em: 575nm Isotype: IgG1 Get better batch-to-batch reproducibility with a recombinant antibody WebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ...

WebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1. WebOct 4, 2024 · The FLAG tags are attached to the N-terminus or C-terminus of the target proteins. The tagged protein binds to the specific monoclonal antibodies conjugated on the matrix of magnetic beads or agarose gel. The residual impurities are washed off using the reagent washing buffer.

WebMonoclonal ANTI-FLAG®M1 antibody produced in mouse is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.colicells. Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

WebKey features and details. Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization. Reacts with: … cube cycles southamptonWebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized … cubed 02 sleeperWebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … cube darwinbox loginWebThis antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Protocols Standard Protocols Click here to view our Standard Protocols Publications All (610) WB … cube cutter for foodWebThe FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. cubed 3d printerWebAntibodies that detect DYKDDDDK Tag can be used in several scientific applications, including Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation and Flow Cytometry. Our DYKDDDDK Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Rat, Goat and … cubed 50WebThe antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG ® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species … eastchester pcr test