WebMar 15, 2016 · The RT-qPCR used a standard super-mix (Bio-Rad SsoFast_EvaGreen SuperMix) for each strain and a standard power-mix (Bio-Rad IQTM Multiplex PowerMix) for Total Bacteria. Opticon Monitor 3 Software and the RT-qPCR Chromo4 (Bio-Rad) were used to perform and analyze the RT-qPCR. WebPtrprimers were designed to target a species-specific multicopy genomic region described previously (Moffat et al., 2015; See et al., 2016). Briefly, a 4.65 kb region of the Ptrisolate Pt-1CBFP carrying the ToxA gene (supercontig_1.4) was aligned to an orthologous genomic region of the PanisolateSN15 (scaffold_55). Three known isolates of the targeted
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Diagnostic real-time RT-PCR for the simultaneous detection of …
Web(Discontinued) iQ™ Multiplex Powermix, 50 x 50 µl rxns, 1.25 ml 1725848 50 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix … WebIq Multiplex Powermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more iQ™ Multiplex Powermix, 200 x 50 µl rxns, 5 ml (4 x 1.25 ml) 1725849 200 x 50 µl reactions, real-time PCR mix for detection of multiple targets in a single tube, 2x mix contains dNTPs, 11 mM MgCl 2, iTaq™ DNA polymerase List Price: Your Price: Log in to see your price Quantity: Add to Cart Add to Quote Add to Hot List Documents Login Required reach rwanda