Lambda terminase
TīmeklisLambda Terminase is an endonuclease encoded by bacteriophage lambda that recognizes and cleaves DNA at cos sites, generating 5′ overhangs of 12 bases … TīmeklisTerminase is composed of two protein subunits, gpNu1 and gpA. The holoenzyme is multifunctional in vitro; it binds to and cleaves lambda DNA at the cos site (where cos represents cohesive-end site), packages DNA into lambda proheads, and is also a DNA-dependent ATPase.
Lambda terminase
Did you know?
TīmeklisTerminase enzymes are common to complex double-stranded DNA viruses and function to package viral DNA into the capsid. We recently demonstrated that the bacteriophage lambda terminase gpA and gpNu1 proteins assemble into a stable heterotrimer with a molar ratio gpA1/gpNu1(2). This terminase protomer possesses … TīmeklisThe terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral …
Tīmeklis2000. gada 20. janv. · It is demonstrated that lambda terminase can be purified as a homogeneous "protomer" species, and a kinetic analysis of the genome maturation and packaging activities of the protomeric enzyme is presented, providing mechanistic insight into the coordinated catalytic activities of terminase enzymes in virus … Tīmeklis2000. gada 29. sept. · Terminase is the enzyme that mediates lambda DNA packaging into the viral prohead. The large subunit of terminase, gpA (641 amino acid residues), has a high-affinity ATPase activity (K(m)=5 microM).
http://www.lambdafaq.org/lambdas-and-closures-whats-the-difference/ TīmeklisThe ability of lambda terminase to cleave cos phi 21 was studied in vitro; lambda terminase cleaved cos phi 21 only 1% as well as it cleaved the phage lambda …
TīmeklisThe role of cosB, the binding site for terminase, the DNA packaging enzyme of bacteriophage lambda, in the nicking reaction. J Mol Biol …
Tīmeklis1997. gada 13. maijs · The terminase enzyme from bacteriophage λ is responsible for excision of a single genome from a concatameric DNA precursor and its insertion into an empty viral procapsid. The enzyme possesses a site-specific endonuclease activity which is responsible for excision of the viral genome and the formation of the 12 … theoretical loss accountingTīmeklis1988. gada 1. jūl. · The large subunit of phage lambda terminase, gpA, the gene product of the phage A gene, interacts with the small subunit, gpNul, to form functional terminase. Terminase binds to lambda DNA at cosB to form a binary complex. The terminase:DNA complex binds a prohead to form a ternary complex. Ternary … theoretical logical grayTīmeklisLambda Exonuclease. DNA specific exonuclease. Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction. Highly processive degradation of double-stranded … theoretical logicTīmeklis2024. gada 10. jūl. · Java supports lambda expressions but not the Closures. A lambda expression is an anonymous function and can be defined as a parameter. The … theoretical logical greyTīmeklis2000. gada 20. janv. · The terminase enzyme from bacteriophage lambda: a DNA-packaging machine. Abstract. This review focuses on the biochemical, biophysical, … theoretical lowest latency on internetTīmeklis2013. gada 25. marts · Terminase enzymes catalyze viral genome packaging. All characterized terminases are composed of large (TerL) and small (TerS) subunits in hetero-oligomeric complexes, although the subunit stoichiometry in the functional motors remains ill defined in most cases (3, 6).Most of the enzymes also possess a DNA … theoretically agree to construct newsTīmeklis2024. gada 10. maijs · In lambda, the large catalytic subunit TerL has N-terminal ATPase and C-terminal nuclease domains involved in phage packaging and maturation. Lambda TerL interacts with the small TerS subunit (gpNu1; NP_040580.1, 181 amino acids) which recognizes the cos site and is involved in stability of the packaging … theoretical loss